Ny publikasjon om XMRV 19 juni 2011: Ingen antigener fra XMRV eller antistoffer ble detektert I denne studien – Alle 148 CFS pasientene var seronegativ for XMRV

En ny rapport om XMRV ble publisert 19 juni 2011: Ingen antigener fra XMRV eller antistoffer ble detektert I denne studien med 148 CFS pasienter. Samtlige var seronegativ for XMRV. Dette var en blindet studie, hvor prøvene var fra en tidligere studie som ble publisert i retrovirology i desember 2010 og konkluderte med kontaminering av prøvene.

Publikasjonen sier blant annet som følger:

 

Failure to detect XMRV‐specific antibodies in the plasma of CFS patients using Highly Sensitive Chemiluminescence Immunoassays

Brendan Oakes1,2, Xiaoxing Qiu3, Susan Levine4, John Hackett Jr.3, Brigitte T. Huber1#

1Department of Pathology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111, USA

2Pharmacology Program, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111, USA

3Infectious Diseases R&D, Abbott Diagnostics, 100 Abbott Park Rd, Abbott Park, IL, 60064, USA

4Private Practice, 115 East 72nd Street, New York, NY, USA

#Corresponding Author

Abstract

In 2009, Lombardi et al. reported their startling finding that the gammaretrovirus xenotropic

murine leukemia virus‐related retrovirus (XMRV) is present in 67% of blood samples of patients suffering from chronic fatigue syndrome (CFS), as opposed to only 3.7% of samples from healthy individuals. However, we and others could not confirm these results, using a nested PCR assay. An alternative to this highly sensitive, but contamination‐prone, technique is to measure the serological response to XMRV. Thus, we tested the plasma samples from our cohorts of CFS patients and healthy controls for the presence of XMRV‐specific antibodies.

Using two novel chemiluminescence immunoassays (CMIAs), we show that none of our samples have any XMRV‐reactive antibodies. Taken together with our previous findings, we conclude that XMRV is not present in any human individual tested by us, regardless of CFS or healthy control.

148 blinded plasma samples from our original CFS and healthy control cohorts were analyzed for the presence of XMRV‐specific antibodies, using the direct format ARCHITECT®p15E and gp70 CMIAs. None of the 148 plasma samples were reactive in the p15E CMIA (Figure 1A). Two of the 148 samples (ID=137, 138) were positive in the gp70 CMIA (Figure 1B). Both specimens were weakly reactive in the gp70 CMIA with sample/cut‐off (S/CO) values of 7.77 (Log N of S/CO = 2.05) and 9.02 (Log N of S/CO = 2.20) respectively. Although the samples were repeat reactive in the gp70 CMIA, they were not reactive by WB. As shown in Figure 2, both samples showed no visible WB bands using either XMRV viral lysate proteins (Figure 2A) or recombinant gp70 protein (Figure 2B).

Publikasjonen datert 19 juni 2011 kan du lese her Oakes et al 2011_19jun2011_XMRV studie

B. Oakes, A. K. Tai, O. Cingoz et al., “Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV‐like sequences,” Retrovirology, vol. 7, no. 109, 2010.

Denne publikasjonen kan du lese her

In our original study, we found no specific relationship between the presence of XMRV

and CFS [17]. However, screening the genomic DNA from peripheral blood lymphocytes of both healthy control and CFS cohorts, we did detect PCR products that were identical to XMRV gag sequences, as well as other MLV gag sequences. Due to the high number of MLV sequences in the mouse genomic DNA, we found it prudent to test for mouse DNA contamination in our samples. Using both a test developed by the Switzer lab at CDC for mouse mitochondrial DNA [14], as well as a test developed by the Coffin lab for the IAP [17], we found that every sample that was positive for XMRV or other MLVs PCR products was also positive for mouse DNA.

Although these data provide an explanation for the detection of MLV sequences in our samples, they do not rule out the possibility that XMRV and mouse DNA contamination could be present in the same sample. To clarify this issue, we tested our plasma samples for the presence of XMRV‐specific antibodies.

Kommentar:

Denne forskningsgruppen har ikke kunnet finne XMRV antigener, som indikerer en immunrespons i sin studie og/eller ved bruk av sin metode.

To av prøvene viste respons, men denne kunne ikke verifiseres ved bruk av Western blot metode.

Denne forskningsgruppen har tidligere forsøkt å finne en link mellom XMRV og ME/CFS, men lykkes ikke med det. De fant kontaminering av prøvene. Vi kjenner disse rapportene som kontamineringsrapportene som ble publisert i desemberutgaven av retrovirology i 2010.

Når det gjelder den tekniske metoden de har anvendt for serologi/antistoff testing av gammaretrovirus, her XMRV og relaterte gammaretrovirus, må vi avvente kommentarer og vurdering fra de som kjenner til denne typen prøver/tester.

Det samme gjelder hvilke pasienter som ble benyttet i denne studien med hensyn til hvilke kriterier som ble brukt, altså om de representerer individer med neuroimmunologisk sykdom.

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